Volume 25 , Issue 2 , December 2023 , Pages 282-292
Solav Sabir Ali Ahmed 1 ; Ridha Hassan Hussein 1
1 Biology Department, College of Sciences, University of Sulaimani, Sulaimani, Kurdistan Region, Iraq
A hormone of theincretin system known as glucagon-like peptide-1 (GLP-1) is important
for a number of glucoregulatory functions. This study aimed to investigate the effect
of inhibitors on the vasorelaxant response of GLP-1 in T1D rats. A single
subcutaneous injection of 50 mg/kg of streptozotocin (STZ) was used to develop
diabetes mellitus. Eighty aorticrings from twenty male albino rats were prepared. An
Automatic Organ Bath was used. In our study, apocynin increased vascular response
to GLP- 1 in the non-diabetic group with (Emax:72.40±0.350) and (pD2: -
9.923±0.444). Ininduced diabetes, the NADPH oxidase inhibitor decreased GLP-1
vasodilatation property with (Emax; 68.91±2.002) and (pD2: -9.480±0.138). The
results show that bisindolylmaleimide IX (RO31-8220) has an increased effect on
GLP-1 vasodilation in non-diabetic groups with (Emax:88.45±38.18) and (pD2: -
10.81±0.281). While in induced diabetic rat’s vascular relaxation of GLP-1
decreased with (Emax: 77.73±2.801) and (pD2: - 10.28±0.203). The data analysis
demonstrated that rotenone with (Emax: 63.69±35.10) (pD2: - 9.612±0.246), and in
diabetic rats with (Emax:69.98±22.94) and (pD2: -9.612±0.246). In our study
oxypurinol with (Emax: 82.16±16.10) and (pD2: - 9.434±0.443), and in diabetic rats
with (Emax: 58.03±8.350) and (pD2: -9.612±0.246). We concluded that inhibitors could
increase the vasorelaxant response of GLP-1 in non-diabetic rats, while this
vasorelaxant response of GLP-1 diminished in diabetic-induced rats