Volume 25 , Issue 2 , December 2023 , Pages 256-262
1 Department of Biology, College of Science, Sulaimani University, Sulaimani City, Kurdistan Region, Iraq
The purpose of this study was to compare the effects of two concentrations of aqueous
crude extract from Ficus carica leaves on fruit fly numbers, sex ratios, and generations,
as well as laboratory albino mouse sperm abnormalities. Fruit flies were given the
concentrations of 1% marked as C1 and 2% marked as C2 in culture media and allowed
to complete their life cycle at an acceptable temperature (25o C±2) in an incubator. Same
concentrations were given to the tested mice in drinking water. Each treatment consisted
of five replications, with untreated mice and flies serving as the control groups. Fruit fly
findings were seen in the first and second generations, and mouse sperm morphology
was examined one month following treatment. The results revealed that
tested concentrations had no significant impact (P≤0.05) on the quantity of fruit flies
mean values were (40.650 ± 2.40 5and 39.250 ± 2.624) froe first and second
concentrations compared to 34.550 ± 2.12 in control group, but they did have a
significant impact (P≤0.05) on the sex ratio (shifted toward females (40.733 ± 1.969) in
comparison to males (35.566 ± 1.838), least significant differences(L.S.D.) were =
5.148, and generation-concentration interactions, the highest number were recorded was
females in second generation (42.900 ± 2.203). The treated mice showed Significant
effect of concentrations on sperm without tail and highly significant effect (P≤0.01) on
sperm without hook (mean values were 5.6 ± 0.37 and 2.0 ± 0.44 in high concentration
while in control group the values were 2.0 ± 0.44 and1.0 ± 0.44) for both aberrations
respectively. Our results clearly indicated change in sex ratio among treated fruit flies
and induction of some morphologic changes in mice sperms after treatment of fig leaves
aqueous crud extract.