Volume 25 , Issue 2 , December 2023 , Pages 1-8
Khelan Saeed Hama Amin 1 ; Karzan Jalal Salih 2 ; Dlzar Dlshad Ghafoor 2
1 Charmo Research Center, Charmo University, Chamchamal, Iraq
2 Medical Laboratory Science Department, Komar University of Science and Technology, Sulaimaniyah, Iraq
Diabetes mellitus type I is an autoimmune disorder in which pancreatic β cell
autoantibodies are the most significant immunological markers. In this study, we
investigated the prevalence of antibodies GADA, IAA, IA2A, and ICA. Seventy-seven
patients were selected for the study and another 93 healthy controls were studied.
Autoantibodies were measured in the serum samples obtained from both patients and the
control group using enzyme-linked immunosorbent assay (ELISA). According to the
results of this study, there was a significant difference in the level of GAD65 when the
patient group was compared to the control. The mean value for the GAD in the control
group was 2.095±0.89, while in patient groups it was 3.56±3.95 ng/ml, and they were
significantly different (p<0.01). A qualitative measurement for both antibodies ICA and
IA2A showed a positive result in more than 50% of the patients while ICA was positive
in 12% of the control groups and IA2A was positive in 1.3% in the control group.
Qualitative assessment of the IAA antibodies revealed that 32.8% were positive, while
all healthy subjects were negative. Fasting C-peptide level in the patient group was
0.745±0.12 ng/ml while it was 2.12±0.48 ng/ml in healthy subjects. HbA1C level in
patients was 10.46±2.27 while it was 5.38±0.24 in healthy subjects. The risk factors,
maternal status, and children’s status effect on the development of diabetes were studied
and it was found that a significant difference (p<0.05) when a family history of DM was
compared with patient and control groups, while there was not significant difference
found between both groups when a family history of other autoimmune diseases was
compared. Neonatal diseases between both groups were compared and no significant
difference was observed as well. From the result of this study, it can be concluded that
environmental risk factors such as obesity, family history of DM may play a significant
role in triggering the immune system and leading to beta cells destruction, while ethnic
background, geography, maternal obesity, maternal diseases, infections during
pregnancy, neonatal diseases such as jaundice, thyroid, vitiligo, and celiac are not
regarded as a potential risk factor in developing the disease. More than 90% of T1D
individuals tested positive for autoantibodies. The most often found autoantibodies were
IA2A and GADA. Antibodies were much more prevalent in female children.