Genetic characterization of peste des petitis ruminants virus (PPRV) from Sulaimani/ Iraq by phylogenetic analysis and sequencing of nucleoprotein and fusion protein gene

Volume 16 , Issue 3 , August 2014 , Pages 49-58

Authors

¹ Molecular diagnostic laboratory, Sulaimani Veterinary directorate, Sulaimani

An outbreak of peste des petitis ruminants (PPR) in sheep was studied in Sulaimani/

Iraqi Kurdistan Region. The study provided the first molecular characterization of the PPRV

lineage associated with fatal PPR infections in small ruminats. RT-PCR was used for diagnosis

of PPRV outbreaks during 2012-2013 and the phylogenetic analysis results based on F and N

genes. It was observed that three tested samples were positive for PPR, from which the two F

genes (ppr/Kurdistan/2012, ppr2/Kurdistan/2012) were genetically close to the (KF478924 &

JF274480) strain (turkey and Egypt) of lineage IV, respectively with 99% nucleotide sequence

homogeneity. Regarding N gene, it was noticed that one sample (ppr3/Kurdistan/2013) where

genetically close to (FJ795511, DQ840197 & DQ840190) strain (Emirate, Saudi Arabia & Israel)

%99, %98 and %97 respectively. The current study suggested that there are at least two

sources of PPRV in Iraq.

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