Phylogenic Analysis and Molecular Characterization of Slemani/Kurdistan/2013 Foot and Mouth Disease Virus Shows Circulation of New Genotype in Iraq

Volume 16 , Issue 3 , July 2014 , Pages 1-11

Authors

¹ Molecular diagnostic laboratory, Sulaimani Veterinary directorate, Sulaimani

Foot-and-mouth disease (FMD) is endemic in Iraq. There were no much informations

about FMD genotypes in Iraq. In this study, FMD type A virus was identified by RT-PCR from

a cow in Kurdistan region. Viral protein1 (VP1) was amplified, sequenced and published in

National Center for Biotechnology Information (NCBI) GenBank as Slemani/Kurdistan/2013.

VP1 nucleic acid and amino acid sequence of Slemani/Kurdistan/2013 was comparatively

analysed with all previously published Iraqi FMDV type A sequences available in NCBI Gen

Bank from 1964 to 2013. The result indicated that Slemani/Kurdistan/2013 had 10 amino acid

residues in immunogenic VP1 region, which were not present in other Iraqi FMD isolates.

Furthermore, sequence alignment of Slemani/Kurdistan/2013 showed the amino acid identity

ranged from 83 % to 93%, and the nucleotide identity ranged from 69% to 93% with other

Iraqi FMDV type A isolates. Phylogenetic tree analysis showed that the identified field isolate

differed from the other Iraqi subtype and could be placed in distinct sub lineages. It can be

concluded that new genotype was circulated in Iraq. Therefore, monitoring the emergence of

new FMDV strains in Iraq is important to enable appropriate vaccines selection.

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