Direct In Vitro Regeneration of Fig (Ficus carica L. CV. Benaty) from Apical and Lateral Buds

Volume 18 , Issue 1 , March 2016 , Pages 77-88

Authors

¹ Department of Horticulture, Faculty of Agriculture, University of Duhok, Iraq

² Ministry of Education, Iraqi Kurdistan Region

Benaty fig plant was successfully propagated by tissue culture technique and a

reproducible and reliable micropropagation protocol was developed through this

investigation. At initiation stage, healthy aseptic cultures from lateral and terminal bud

explants of about 85% were achieved when the explants were treated with 0.15% HgCl 2

for 7 minutes. A combined solution of ascorbic acid and citric acid (100 and 150 mgl -1 )

was successfully used to overcome the problem of phenolic compounds exudation from

the explants into the culture media. At shoot multiplication stage, the addition of 2.0 mgl -

1 from both BA and kinetin gave the highest number of shoots per explant estimated at

2.66 and 3.33 shoots/ explant respectively. As well as, activated charcoal promoted

shoots multiplication in all multiplication parameters. The highest number of shoots per

explants (5 shoots/ explant) was obtained on MS medium containing 4.0 gl -1 AC. At root

formation stage, the interaction treatment of full MS salt strength with the use of 0.25

mgl -1 NAA gave the highest rooting percentage reaching 87.5%. The highest number of

roots (18.5 roots/ explant) was recorded as well from the combined treatment of full MS

salt strength with the use of 0.25 mgl -1 NAA. While the longest roots (6.66 cm) were

recorded from the interaction treatment of half salt strength and 0.50 mgl -1 IBA. The

addition of activated charcoal to the rooting culture medium decreased the rooting

percentage from 87.5% at control treatment to 50% and 62.5% when 2.0 and 4.0 gl -1 AC

were respectively added. The in vitro propagated fig plantlets were gradually moved in a

successful way from lab to field conditions with 95% survival rate.

Statistics