Identification of Animal Species in Meat Broth by Simplex and Multiplex PCR

Volume 16 , Issue 1 , February 2014 , Pages 97-102

Authors

¹ Molecular diagnostic laboratory, Sulaimani Veterinary directorate, Sulaimani

In some country, some restaurants may use fraudulent substitution in meat product that has

less commercial value or undesirable. Effective laboratory tests must be available for prevention

of this phenomenon. In this study meat broth sample was successfully used to identify seven

animal species in cooked meals by simplex and multiplex PCR using species specific primers for

conserved region of mitochondrial DNA (mtDNA). The results showed effective amplification of

mtDNA without cross reaction between primers. Whereas existing methods require mincing and

homogenization of direct meat samples, this meat broth sampling method can be performed

directly and is thus easier to perform. In conclusion, meat broth sample could be used instead of

direct meat tissues for species identification of cooked meat in restaurants to control

adulteration substitutions of undesirable meat and protection of the customer rights

Statistics