Determination of tetracycline hydrochloride in some pharmaceutical products using flow injection spectrophotometric technique

Volume 18 , Issue 1 , March 2016 , Pages 1-12

Authors

¹ Chemistry Department, College of Education Science, Salahaddin University-Hawler, Iraqi-Kurdistan Region

A simple, rapid, and sensitive flow injection spectrophotometric system for

determination of tetracycline hydrochloride (TCH) in pharmaceutical

products. The method is based on diazotization of 2-aminopyridine and

subsequent coupling with tetracycline hydrochloride in alkaline medium. The

formed azo-dye showed maximum absorbance at 388 nm and the calibration

curve in the linear range of 25.0-150.0 µg/ml of tetracycline hydrochloride

with correlation coefficient 0.9988, while the detection limit was 10 µg/ml

and sample frequency 123 samples/hour. The accuracy and precision data for

determination of TCH by flow injection analysis method for triplicate injection

at three concentration levels(25, 80 and 150 µg/ml) the relative standard

deviation (RSD %) are 1.04, 0.247 and 1.75%, and relative error percent (E rel )

are -3.92, +1.06 and -1.9%. The proposed method was successfully applied to

the determination of tetracycline hydrochloride in pharmaceutical products.

The results obtained were in good agreement with those obtained by

reference method.

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