Isolation, Molecular Identification and in vitro Antibiotic Susceptibility Testing of Mycoplasma agalactiae From Goats in Two Provinces of Kurdistan Region-Iraq.

Volume 17 , Issue 3 , September 2015 , Pages 127-136

Authors

¹ University of Sulaimania - College of Veterinary Medicine, department of clinic and internal medicine

Mycoplasma agalactiae is one of the causal agents of classical contagious agalactia

(CA). It occurs in many parts of the world and most notably in the Mediterranean

Basin. The aims of this study were isolation and molecular identification of

Mycoplasma agalactiae for the first time in Kurdistan region from goats showing

contagious agalactia and in vitro evaluation of the activities of different

antimicrobial against Mycoplasma agalctiae. During the period of January 2011 to

November 2012 a total of 126 milk samples were collected (68 from Slemani

governorate and 58 from Arbil governorate) from goats that had clinical signs of

CA. Mycoplasma agalactiae was recovered from 102(81%) milk samples out of

(126) divided as; 58 (85.3%) out of 68 milk samples in Slemani governorate were

positive for M. agalactiae while 44(75.9%) out of 58 milk samples were positive in

Arbil governorate. All isolates were confirmed by amplification of 1624 bp uvrC

gene by PCR assay. GenBank accession number of the nucleotide and amino acid

sequences of Slemani and Arbil isolates were reported in this study is (KC 594646),

(KC 594647) respectively. The isolates in current work from both governorates

were showed 99% homology to each other and the topology of the phylogenetic

tree indicated that both field isolates were clustered together and they were

belonging to sub linage that contain most of PG2 strains. Danofloxacine and

Azithromycine showed a great effectiveness against all isolates and may be

considered as standard treatment for CA in this region.

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