Antibiotic Resistance Pattern and Detection of Some ESBL Genes amongDiarrheagenic E. coli O157:H7.

Volume 17 , Issue 3 , September 2015 , Pages 111-126

Authors

¹ Department of Biology, College of Education, Salahaddin University – Hawler, Iraq

During the period of 20th of May to1st of November 2013, twenty five isolatesof E. coli

O157:H7 were identified among 200 samples taken from children under ten years old

suffer from diarrhea (68 males and 132 females) admitted to Rapareen Pediatric hospitals

in Erbil City. Isolates were identified from stool sample by using cultural, morphological,

biochemical characteristics and serological test. Antibiotic sensitivity testing was done

for all isolates by using fourteen antibiotic types The resistance rate of the isolates

toward these antibiotics were 100 % for AMP, 96% for MET, 76% for CTX, 72% for TE

and KF, 68% for TMP, 56% for FEP, 52% for C and SXT, 48% for CRO, 28% for AK,

20% for CIP, while the lowest percent 16% was for CN, and all isolates were sensitive

for IPM. Extended spectrum ß-lactamase (ESBL) production was done for all isolates

.Out of 25 E. coli O157:H7isolates, 8(32%) were ESBL producers, while17(68%) were

non ESBL producers. The plasmid profile of 25 isolates conducted by using gel

electrophoresis, the results revealed different bands with molecular weight ranging

between (2kbp- more than 10kbp) in size. The transformation process conducted

successfully for (E1 and E15) and the results showed that the resistance genes for

ampicillin, Cephalothin, Cefepime, metronidazole and trimethoprimwere located on

plasmid DNA for E1 isolate, while for E15 isolate, the ampicillin, Cephalothin,

Cefepime, ceftriaxone, Cefotaxime, metronidazole and trimethoprimlocated on plasmid.

The results of transformation confirmed by gel electrophoresis, and showed that one

plasmids of E1 and two plasmid E15 had been transformed successfully with molecular

weight of more than 10 Kbp. All E. coli O157:H7isolates were screened for the presence

of ESBL genes including (blaCTX-M - blaSHV-and blaTEM) on plasmid DNA using

polymerase chain reaction (PCR) assay. Results showed that all isolates were positive for

the existence of ( blaSHV-and blaTEM), while 88% of the isolates contained( blaCTXM) .

Statistics