Protective Effects of Exogenous Melatonin on Cisplatin- Induced Acute Nephrotoxicity in Rats

Volume 19 , Issue 1 , March 2017 , Pages 17-26

Authors

¹ Department of General Science, College of Basic Education, Charmo University, Kurdistan Region, Iraq

² Department of Biology, School of Sciences, University of Sulaimani, Kurdistan Region, Iraq

This study was planned to investigate the consequences of exogenous administration of

melatonin on the amelioration of some biochemical variables in cisplatin (CP)-induced

acute nephrotoxicity in rats. Twenty-four male rats weighing 300-350g were used.

Housing of the animals and the experiment were achieved and the rats were -housed in

the Department of Biology /College of Science/ Sulaimani University. The Animals were

divided into three groups (n=5); control group (receiving prepared standard chow for rats

and water ad libitum); model group(The animals administered a single intraperitoneal

(i.p) dose of CP (8 mg/kg BW.); and the third group in addition to CP injection the

animals were supplemented with melatonin (180 mg/kg food) at the same day of CP-

injection for five days. Finally, the blood specimen was taken using cardiac puncture.

The studied parameters include Malondialdehyde (MDA), serum creatinine, serum urea,

serum total bilirubin, serum albumin, serum electrolytes (Ca+2, Na+ and K+), as well as

some hematological parameters (WBC, RBC, HCT, HGB). Our results showed

significant (P<0.05) elevation in the serum level of MDA,, creatinine, urea and total

bilirubin in model group. On the other hand, the WBC count was also increased

significantly (P<0.01), whereas there was no significant alterations in the level of

calcium, sodium, and potassium. The outcome of our study indicated that the melatonin

administration in injected rats for five days significantly (P<0.01) improved the elevated

serum levels of MDA, creatinine, urea, total bilirubin and WBC count. In conclusion,

this study confirmed that melatonin has protective effects of ameliorating the

nephrotoxicity induced by cisplatin in rat.

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