Flow- Injection Spectrophotometric Determination of some Sulpha Drugs via Oxidative Coupling with 4-Amino-N, N-diethylaniline. Application to Various Samples

Volume 12 , Issue 1 , January 2009 , Pages 67-76

Authors

Yousif J. Azeez 1

1 College of Science, Salahaddin University

DOI logo 10.17656/jzs.10196

Keywords

Abstract

A flow injection spectrophotometric method is described for the determinaiton of sulphonamides

in aqueous and biological samples. The method is based on the oxidative coupling reaction of 4-amino-N,

N-diethylaniline with sulphonamide drugs in the presence of potassium dichromate as oxidizing agent.

Intense violet, water soluble dye was obtained which has a maximum absorption at 550nm,. Linear

calibration graphs were obtained for different sulphonamides with a molar absorpitivity of 4200-5270 l mol-1 cm-1

and a relative standard deviation less than 0.56%. The proposed method has proved to be highly sensitive (detection limit range 0.30-0.63 μg ml-1  sulphonamide) and accurate (recovery % was 98.2-101.2%). The nature of the coloured dye and its stability constant were determined. The method was applied successfully to the determination of sulphonamide drugs in pharmaceutical preparations, blood and urine samples.Keywords: FIA, Spectrophotometer 4-amino-N, N-diethylaniline

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