Molecular Characterization for Karadi Sheep Breed Using Random Amplified Polymorphic DNA Markers

Volume 16 , Issue 1 , October 2014 , Pages 231-243

Authors

¹ Dept. of Animal Resource, College of Agriculture, University of Salahaddin

² Dept. of Animal Production, Faculty of Agricultural Science, University of Sulamania

RAPD-DNA markers were used to study genetic characterization among four

locations for Karadi sheep breed in Sulamania governorate. A total of 40 samples were

typed using twenty RAPD primers. Nine out of the twenty primers had clear bands,

which used to investigate the genetic variations among four locations(Halabjai Taza,

Penjwen, Halabja and Sharbazer) of same breed. Out of the nine primers 8 of them are

polymorphisms. A total of 119 bands were scored, of which 27 bands (25.05%) were

polymorphic and seven of polymorphic band were unique bands. For all location, Nei’s

gene diversity, Shannon index and percentage of polymorphic loci are respectively

averaged of 0.2361, 0.3415 and 34.60. Using unweighted pair-group method with

arithmetic average (UPGMA) dendrogram, the three clusters, the 1st cluster branch

consisted of the Karadi sheep in location 1 and 3, the 2nd cluster was including Karadi

sheep from location 2 and the 3rd one including sheep from location 4. These results

indicated that the Karadi sheep in location 1 and 3 is most genetically distant from the

Karadi sheep in location 2 .The dendrograms show that there are moderately genetic

diversity among Karadi sheep breed, were ranged from 0.251to 0.541. Based on the high

degree of genetic distance among the four locations it is concluded that there are widely

area for selection in this breed of sheep.

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