DNA profiling of F1 Hybrid in Bambara Groundnut (Vigna subterranea L. Verdc.) using Simple Sequence Repeat Marker

Volume 16 , Issue 1 , October 2014 , Pages 85-94

Authors

¹ Field Crops Department, Faculty of Agricultural Sciences, Sulaimani University

Bambara groundnut (Vigna subterranea L. Verdc.) is an indigenous crop of

African origin. It is considered as a drought tolerant and the third most important

leguminous crop in Sub-Saharan Africa due to its agronomic and nutritional potential.

Screening of presumed hybrids and progenies with molecular marker proved to be more

accurate and reliable compared to morphological characterizations. Testing the

accuracy of presumed hybrids resulted from the cross of African and Indonesia

landraces were performed using 12 SSR markers. Out of five crosses only one hybrid

resulted from the cross BC x S19 was confirmed to be genuine with four polymorphic

markers. The seeds of other crosses were found to come from the self-pollination of

maternal parent. These results clearly demonstrate that SSR markers should be useful

for rapid identification of true hybrid at the early stage of growth. Hence, the technique

contributes to genotype the progenies more accurately to develop genetic mapping and

QTL analysis, aiming towards more efficient utilization, conservation and development

of bambara groundnut germplasm.

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