Purification and Immobilization of Amylase Enzyme Produced from Local Isolate of Bacillus subtilis

Volume 7 , Issue 1 , January 2004 , Pages 61-68

Authors

¹ College of Science, University of Sulaimani

Amylase enzyme produced from B. subtilis was purified by two main steps included

precipitation with ammonium sulfate 40-80% saturation and gel filtration chromatography on

Sephadex colunm (G-100). The obtained purification foid and recovery werc (67.92) and (43.9) %

respectively.

The ensyme was immobilized by enh'apment in calcium alginate beacls. The enzyme retained

53% af it's origiaal activity after a month of storage at 4oC. On the other hand, free enzyme lost its

activity completely in less than 20 days. These results were revealed the possibiliry of using pwified

amylase in indusky fields. Results also indicate the efficiency of immobilization technique by caicium

alginate beads and preserving of the enzyme activity fbr a relatively long period of time.

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